Molecular profiling of the Clostridium leptum subgroup in human fecal microflora by PCR-denaturing gradient gel electrophoresis and clone library analysis

Article Abstract:

A PCR-based denaturing gradient gel electrophoresis (DGGE) method was developed to investigate the diversity of Clostridium leptum subgroup in human fecus. The results show that DGGE combined with clone library analysis is an effective method for monitoring and analyzing the composition of this important population in the human gut flora.

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Molecular characterization of Clostridium tetani strains by pulsed-field gel electrophoresis and colony PCR

Article Abstract:

Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among 5 strains tested, one turned out to contain a mixture of two genetically different clones and two to contain bacteria differing by the presence of the 74-kb plasmid harboring the tetanus toxin gene.

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Development and validation of PCR primers to assess the diversity of Clostridium spp. in cheese by temporal temperature gradient gel electrophoresis

Article Abstract:

The nested-PCR temporal temperature gradient gel electrophoresis (TTGE) approach for the detection of bacteria belonging to phylogenetic cluster I of the genus Clostridium was developed. The results demonstrated the efficacy suggesting the involvement of this species in butyric acid fermentation.

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